d609 tocris bioscience Search Results


d609  (Tocris)
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fipi  (Bio-Techne corporation)
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2ohoa (200 m)  (Tocris)
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vin m2  (Cayman Chemical)
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Cayman Chemical vin m2
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bapta-am  (Biomol GmbH)
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ethylacetate  (Millipore)
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Millipore ethylacetate
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imipramine hydrochloride  (Nacalai)
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cid 755673  (Bio-Techne corporation)
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edelfosine (cas 77286-66-9)  (Tocris)
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cytochalasin d (cd) (for actin polymerization)  (Millipore)
90
Millipore cytochalasin d (cd) (for actin polymerization)
Inhibition of bacterial uptake blocks PGE2 biosynthesis by inoculated macrophages. (a) BMDMs were pretreated with 10 μM <t>cytochalasin</t> <t>D</t> (Cyto D) and then inoculated with F. tularensis LVS at an MOI of 200:1, and PGE2 in the supernatant was quantified by enzyme-linked immunosorbent assay 24 hours after inoculation (n = 3). (b and c) BMDMs (b) or RAW 264.7 macrophages (c) pretreated with inhibitors or vehicle and then inoculated with F. tularensis at an MOI of 200:1. At 6 and 24 hours after inoculation, macrophages were lysed and intramacrophage bacteria were quantified (n = 3). The asterisk denotes a statistical difference compared to the untreated F. tularensis-infected group (P ≤ 0.05). Graphs are means of pooled experiments ± SEMs.
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Inhibitors used in this study a

Journal: Infection and Immunity

Article Title: Francisella tularensis LVS Induction of Prostaglandin Biosynthesis by Infected Macrophages Requires Specific Host Phospholipases and Lipid Phosphatases

doi: 10.1128/IAI.02060-14

Figure Lengend Snippet: Inhibitors used in this study a

Article Snippet: The inhibitors used in these studies were pyrrophenone (for cPLA 2 ), CAY10590 (for sPLA 2 ), Edelfosine (for phosphatidylinositol [PI]-PLC), JZL184 (for MAGL), and indomethacin (for COX-2) (all purchased from Cayman Chemical); propranolol (for PAP), RHC80267 (for DAGL), MJ33 (for iPLA 2 ), U73122 (for PLC), and cytochalasin D (CD) (for actin polymerization) (all purchased from Sigma-Aldrich); VU0155056 (for PLD1/2) (purchased from Avanti Polar Lipids, Inc.); and D609 (for phosphatidylcholine [PC]-PLC) (purchased from Tocris Bioscience).

Techniques:

Inhibition of bacterial uptake blocks PGE2 biosynthesis by inoculated macrophages. (a) BMDMs were pretreated with 10 μM cytochalasin D (Cyto D) and then inoculated with F. tularensis LVS at an MOI of 200:1, and PGE2 in the supernatant was quantified by enzyme-linked immunosorbent assay 24 hours after inoculation (n = 3). (b and c) BMDMs (b) or RAW 264.7 macrophages (c) pretreated with inhibitors or vehicle and then inoculated with F. tularensis at an MOI of 200:1. At 6 and 24 hours after inoculation, macrophages were lysed and intramacrophage bacteria were quantified (n = 3). The asterisk denotes a statistical difference compared to the untreated F. tularensis-infected group (P ≤ 0.05). Graphs are means of pooled experiments ± SEMs.

Journal: Infection and Immunity

Article Title: Francisella tularensis LVS Induction of Prostaglandin Biosynthesis by Infected Macrophages Requires Specific Host Phospholipases and Lipid Phosphatases

doi: 10.1128/IAI.02060-14

Figure Lengend Snippet: Inhibition of bacterial uptake blocks PGE2 biosynthesis by inoculated macrophages. (a) BMDMs were pretreated with 10 μM cytochalasin D (Cyto D) and then inoculated with F. tularensis LVS at an MOI of 200:1, and PGE2 in the supernatant was quantified by enzyme-linked immunosorbent assay 24 hours after inoculation (n = 3). (b and c) BMDMs (b) or RAW 264.7 macrophages (c) pretreated with inhibitors or vehicle and then inoculated with F. tularensis at an MOI of 200:1. At 6 and 24 hours after inoculation, macrophages were lysed and intramacrophage bacteria were quantified (n = 3). The asterisk denotes a statistical difference compared to the untreated F. tularensis-infected group (P ≤ 0.05). Graphs are means of pooled experiments ± SEMs.

Article Snippet: The inhibitors used in these studies were pyrrophenone (for cPLA 2 ), CAY10590 (for sPLA 2 ), Edelfosine (for phosphatidylinositol [PI]-PLC), JZL184 (for MAGL), and indomethacin (for COX-2) (all purchased from Cayman Chemical); propranolol (for PAP), RHC80267 (for DAGL), MJ33 (for iPLA 2 ), U73122 (for PLC), and cytochalasin D (CD) (for actin polymerization) (all purchased from Sigma-Aldrich); VU0155056 (for PLD1/2) (purchased from Avanti Polar Lipids, Inc.); and D609 (for phosphatidylcholine [PC]-PLC) (purchased from Tocris Bioscience).

Techniques: Inhibition, Enzyme-linked Immunosorbent Assay, Infection